Fig. 3
From: High-fat diet-induced osteoporosis in mice under hypoxic conditions
The effects of a HFD on the femurs, colon, and adipose tissue of mice under hypoxic conditions. Note: (A) Micro-CT scan image of femur; (B) bone volume to tissue volume ratio (BV/TV, %); (C) bone surface to tissue volume ratio (BS/TV, mm− 1); (D) trabecular number (Tb.N, mm− 1); (E) trabecular spacing (Tb.Sp, mm); (F) trabecular thickness (Tb.Th, mm); (G) cortical thickness (Ct.Th, mm); (H) pore volume (Po.V, mm3); (I) HE staining of adipose tissue (scale bar = 40 μm); (J) HE staining of colon tissue (longitudinal section: scale bar = 40 μm; cross section: scale bar = 200 μm); (K) HE staining of femur tissue (scale bar = 200 μm); (L) TRAP staining of femur tissue (scale bar = 20 μm); (M) Number of osteoclasts in femoral tissue.The data are presented as the means ± SDs (A-H: n = 6; I-M: n = 3). *P < 0.05 and **P < 0.01 indicate statistically significant differences compared with the control group