Fig. 5

FHL2 knockdown suppressed apoptosis and promoted autophagy in IL-1β induced chondrocytes, and downregulation of FHL2 inhibited both apoptosis and autophagy in chondrocytes without IL-1β stimulation. (A) The protein expressions of BAX, BCL-2, Caspase-3, and Caspase-7 were detected by WB. (B) Apoptotic rates of different groups were determined by flow cytometric analyses. (C) The levels of Beclin1 in protein expression and the ratio of LC3-II/LC3-I were detected by WB. The data are presented as the mean ± SD. Significant differences between groups were determined by Student’s t-test; * P < 0.05, **P < 0.01, ***P < 0.001; n = 3