Figure 4

Stressed-matrix contraction of primary DD cultures. The graph displays the percent FPCL contraction of five patient normal/control (C) and diseased (D) matched primary cell lines over an incubation period of 5 days. Data plotted represents the mean ± SEM (standard error of the mean) values of all five patient-matched primary cell lines. Representative images of contracting FPCLs are shown in the lower panel for the indicated time points after mechanical release of the FPCLs. Digital images of the contracting FPCLs were analysed using NIH Imaging software to determine lattice areas for the indicated time points. Statistical pair-wise analysis was performed using a student t-test analysis. *Statistical significance (P < 0.05) between disease and control FPCLs.